Structure of the Constant and 3′ Untranslated Regions of the Murine γ2b Heavy Chain Messenger RNA
- 14 December 1979
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 206 (4424), 1299-1303
- https://doi.org/10.1126/science.117548
Abstract
The complete coding sequence for the constant region of the mouse gamma 2b immunoglobulin heavy chain and the 3' untranslated region has been determined. The coding portion of the sequence is 1008 nucleotides long (amino acid residues 114 to 449), and the 3' noncoding region contains 102 nucleotides preceeding the polyadenylate. An extra carboxyl-terminal lysine residue which had not been observed in the gamma 2b or other gamma subclass protein sequences occurs in the nucleotide sequence and is probably processed posttranslationally. A 17-nucleotide sequence occurs with slight variation twice in CH1 and once in CH2 domains in the same relative location but with different translational phase. This sequence may be the site of crossover in a gamma 2b . gamma 2a heavy chain variant, an indication of possible recombinational activity of some kind.Keywords
This publication has 20 references indexed in Scilit:
- Domains and the hinge region of an immunoglobulin heavy chain are encoded in separate DNA segmentsNature, 1979
- The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cellsCell, 1978
- Complete sequence of constant and 3′ noncoding regions of an immunoglobulin mRNA using the dideoxynucleotide method of RNA sequencingCell, 1978
- An IgG2a-producing variant of an IgG2b-producing mouse myeloma cell line. Structural studies on the Fc region of parent and variant heavy chainsBiochemistry, 1978
- Isolation, purification, and properties of mouse heavy-chain immunoglobulin mRNAsBiochemistry, 1978
- Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormoneNature, 1977
- The primary structure of rabbit β-globin mRNA as determined from cloned DNACell, 1977
- Nucleotide sequence of bacteriophage φX174 DNANature, 1977
- GlycoproteinsAnnual Review of Biochemistry, 1972
- Variability of Interchain Binding of Immunoglobulins: Interchain Bridges of Mouse IgG2a and IgG2bNature, 1970