Analysis of Expressed Sequence Tag Loci on Wheat Chromosome Group 4
- 1 October 2004
- journal article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 168 (2), 651-663
- https://doi.org/10.1534/genetics.104.034827
Abstract
A total of 1918 loci, detected by the hybridization of 938 expressed sequence tag unigenes (ESTs) from 26 Triticeae cDNA libraries, were mapped to wheat (Triticum aestivum L.) homoeologous group 4 chromosomes using a set of deletion, ditelosomic, and nulli-tetrasomic lines. The 1918 EST loci were not distributed uniformly among the three group 4 chromosomes; 41, 28, and 31% mapped to chromosomes 4A, 4B, and 4D, respectively. This pattern is in contrast to the cumulative results of EST mapping in all homoeologous groups, as reported elsewhere, that found the highest proportion of loci mapped to the B genome. Sixty-five percent of these 1918 loci mapped to the long arms of homoeologous group 4 chromosomes, while 35% mapped to the short arms. The distal regions of chromosome arms showed higher numbers of loci than the proximal regions, with the exception of 4DL. This study confirmed the complex structure of chromosome 4A that contains two reciprocal translocations and two inversions, previously identified. An additional inversion in the centromeric region of 4A was revealed. A consensus map for homoeologous group 4 was developed from 119 ESTs unique to group 4. Forty-nine percent of these ESTs were found to be homoologous to sequences on rice chromosome 3, 12% had matches with sequences on other rice chromosomes, and 39% had no matches with rice sequences at all. Limited homology (only 26 of the 119 consensus ESTs) was found between wheat ESTs on homoeologous group 4 and the Arabidopsis genome. Forty-two percent of the homoeologous group 4 ESTs could be classified into functional categories on the basis of blastX searches against all protein databases.Keywords
This publication has 38 references indexed in Scilit:
- Group 3 Chromosome Bin Maps of Wheat and Their Relationship to Rice Chromosome 1Genetics, 2004
- A 2600-Locus Chromosome Bin Map of Wheat Homoeologous Group 2 Reveals Interstitial Gene-Rich Islands and Colinearity With RiceGenetics, 2004
- Development of an Expressed Sequence Tag (EST) Resource for Wheat (Triticum aestivum L.)Genetics, 2004
- The Deletion Stocks of Common WheatJournal of Heredity, 1996
- Structural evolution of wheat chromosomes 4A, 5A, and 7B and its impact on recombinationTheoretical and Applied Genetics, 1995
- Chromosome structure of durum wheatTheoretical and Applied Genetics, 1990
- Gametocidal chromosomes and their induction of chromosome mutations in wheat.The Japanese Journal of Genetics, 1990
- RFLP-based genetic maps of wheat homoeologous group 7 chromosomesTheoretical and Applied Genetics, 1989
- Inferred chromosome morphology of the ancestral genome ofTriticumÖsterreichische botanische Zeitschrift, 1984
- Chromosomal interchanges and the phylogeny of wheatHeredity, 1967