Subcellular Localization of Merocyanine 540 (MC540) and Induction of Apoptosis in Murine Myeloid Leukemia Cells ¶
- 1 May 2007
- journal article
- Published by American Society for Photobiology in Photochemistry and Photobiology
- Vol. 72 (1), 114-120
- https://doi.org/10.1562/0031-8655(2000)0720114slomma2.0.co2
Abstract
Subcellular localization of photosensitizers is thought to play a critical role in determining the mode of cell death after photodynamic treatment (PDT) of leukemia cells. Using confocal laser scanning microscopy and fluorescent organelle probes, we examined the subcellular localization of merocyanine 540 (MC540) in the murine myeloid leukemia M1 and WEHI 3B (JCS) cells. Two patterns of localization were observed: in JCS cells, MC540 was found to localize on the plasma membrane and mitochondria; and in M1 leukemia cells, MC540 was found to localize on lysosomes. The relationship between subcellular localization of MC540 and PDT‐induced apoptosis was investigated. Apoptotic cell death, as judged by the formation of apoptotic nuclei, was observed 4 h after irradiation in both leukemia cell lines. Typical ladders of apoptotic DNA fragments were also detected by DNA gel electrophoresis in PDT‐treated JCS and M1 cells. At the irradiation dose of 46 kJ/m2 (LD90 for JCS and LD86 for M1 cells), the percentage of apoptotic JCS and M1 cells was 78 and 38%, respectively. This study provided substantial evidence that MC540 localized differentially in the mitochondria, and the subsequent photodamage of the organelle played an important role in PDT‐mediated apoptosis in myeloid leukemia cells.Keywords
This publication has 32 references indexed in Scilit:
- A Comparison of the Photodynamic Effects of Temoporfin (mTHPC) and MC540 on Leukemia Cells: Efficacy and ApoptosisPhotochemistry and Photobiology, 1998
- Photodynamic TherapyJNCI Journal of the National Cancer Institute, 1998
- A study of the binding of merocyanine 540 to myeloid leukemia M1 cells using an intensified charge-coupled device for fluorescence imaging microscopyJournal of Photochemistry and Photobiology B: Biology, 1997
- The Role of Subcellular Localization in Initiation of Apoptosis by Photodynamic TherapyPhotochemistry and Photobiology, 1997
- Apoptosis or Necrosis Following Photofrin® Photosensitization: Influence of the Incubation ProtocolPhotochemistry and Photobiology, 1996
- Rapid Initiation of Apoptosis by Photodynamic TherapyPhotochemistry and Photobiology, 1996
- Factors affecting the amount and the mode of merocyanine 540 binding to the membrane of human erythrocytes. A comparison with the binding to leukemia cellsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1995
- New trends in photobiology: Photosensitizers as virucidal agentsJournal of Photochemistry and Photobiology B: Biology, 1993
- Merocyanine 540 Sensitized Photoinactivation of Enveloped Viruses and Its Application in the Sterilization of Blood ProductsPublished by Springer Science and Business Media LLC ,1990
- MEROCYANINE 540Photochemistry and Photobiology, 1987