Temperature-Sensitive Nonsense Mutations in Essential Genes of Escherichia coli
- 1 December 1973
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 116 (3), 1336-1342
- https://doi.org/10.1128/jb.116.3.1336-1342.1973
Abstract
Cells containing nonsense mutations in essential genes have been isolated in a strain of Escherichia coli that carried the su4ts gene which specifies a temperature-sensitive tyrosine transfer ribonucleic acid. Such cells are unable to form colonies at temperatures which inactivate this suppressor transfer ribonucleic acid. A screening procedure for the identification of mutants that carry temperature-sensitive nonsense mutations in essential genes is described, and certain properties of two such mutants are reported.Keywords
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