The production of foot-and-mouth disease virus from BHK 21 C 13 cells grown on the surface of DEAE sephadex A50 beads
- 1 May 1976
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 18 (5), 659-667
- https://doi.org/10.1002/bit.260180506
Abstract
Methods are described which make possible the production of foot-and-mouth disease (FMD) virus from BHK 21 C13 monolayer cells which have been grown on the surface of serum coated DEAE Sephadex A50 beads. The yield of cells and their susceptibility to infection by FMD virus are equivalent to conventional Roux monolayer systems. The potential for the commercial application of the DEAE Sephadex A50 system is discussed in relation to other unit process monolayer systems and in particular to the system in which cells are cultured in a deep bed of small glass spheres.This publication has 10 references indexed in Scilit:
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