ERYTHROID BURST-PROMOTING ACTIVITY OF PURIFIED RECOMBINANT HUMAN GM-CSF AND INTERLEUKIN-3 - STUDIES WITH ANTI GM-CSF AND ANTI IL-3 SERA AND STUDIES IN SERUM-FREE CULTURES
- 1 October 1988
- journal article
- research article
- Vol. 72 (4), 1381-1386
Abstract
We studied the erythroid burst-promoting activity (BPA) of recombinant human granulocyte/macrophage colony-stimulating factor (GM-CSF) and Interleukin-3 (IL-3) with two experimental approaches. First we studied the effects of polyclonal antisera prepared against human GM-CSF and gibbon IL-3 on colony formation from 1,000 bone marrow null cells/dish in serum-containing culture. Both GM-CSF and IL-3 independently enhanced erythroid burst formation; however, IL-3 showed more BPA activity than GM-CSF. These data are in agreement with an emerging view that the primary targets of IL-3 are primitive progenitors and that the targets of GM-CSF are intermediate progenitors, including erythroid burst-forming units (BFU-E). The proliferation of one population of BFU-E was independent of GM-CSF or IL-3. To characterize this population of BFU-E further, we developed a serum-free culture assay for the purified progenitors by incorporating insulin-like growth factor-1 (IGF-1) to the serum-free medium. The development of erythroid bursts was supported by IL-3, IGF-1, and erythropoietin (Ep) in a serum-free culture system and to a lesser extent by the combination of GM-CSF, IGF-1, and Ep. Although the burst-promoting ability of GM-CSF and IL-3 was again demonstrated in this system, unlike serum-containing culture Ep alone did not support burst formation. These results indicate that when fetal calf serum (FCS) is present, the culture system contains BPA that is not GM-CSF or IL-3.This publication has 31 references indexed in Scilit:
- Interactions of insulin, insulinlike growth factor II, and platelet-derived growth factor in erythropoietic culture.JCI Insight, 1985
- Interleukin 3 promotes erythroid burst formation in "serum-free" cultures without detectable erythropoietin.Proceedings of the National Academy of Sciences of the United States of America, 1985
- Colony formation by primitive hemopoietic progenitor cells in serum-free medium.Proceedings of the National Academy of Sciences of the United States of America, 1985
- ERYTHROID PROGENITORS IN POLYCYTHEMIA-VERA - DEMONSTRATION OF THEIR HYPERSENSITIVITY TO ERYTHROPOIETIN USING SERUM FREE CULTURES1982
- Complete replacement of serum in primary cultures of erythropoietin-dependent red cell precursors (CFU-E) by albumin, transferrin, iron, unsaturated fatty acid, lecithin and cholesterolExperimental Cell Research, 1980
- Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes.The Journal of Experimental Medicine, 1978
- THE ROLE OF ERYTHROPOIETIN IN REGULATION OF POPULATION SIZE AND CELL CYCLING OF EARLY AND LATE ERYTHROID PRECURSORS IN MOUSE BONE MARROWCell Proliferation, 1977
- Erythroid colony formation in cultures of human marrow: Effect of leukocyte conditioned mediumJournal of Cellular Physiology, 1977
- ERYTHROPOIETIC PRECURSORS IN MICE UNDER ERYTHROPOIETIC STIMULATION AND SUPPRESSION1977
- Erythropoietin sensitivity as a differentiation marker in the hemopoietic system: Studies of three erythropoietic colony responses in cultureJournal of Cellular Physiology, 1976