A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids
- 1 August 1967
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 104 (2), 627-633
- https://doi.org/10.1042/bj1040627
Abstract
1. An acid ninhydrin reagent was found to react specifically in forming a pink product (E(max.) 560mmu) with cysteine. 2. The method was highly sensitive for the determination of cysteine (in 28.0x10(3)). Homocysteine, glutathione, proline, ornithine and other naturally occurring amino acids tested did not give a similar reaction. 3. The reaction product was stable for at least 3-4hr. at room temperature and the extinction was proportional to the concentration in the range 0.05-0.5mumole of cysteine. 4. The acid ninhydrin reagent also gave yellow products (E(max.) 370-404mmu) with tryptophan, 5-hydroxytryptophan, 5-hydroxytryptamine and indol-3-ylacetic acid. 5. The method was applied for the determination of cysteine in perchloric acid extracts of rat brain, liver and blood.Keywords
This publication has 6 references indexed in Scilit:
- Dithiothreitol, a New Protective Reagent for SH Groups*Biochemistry, 1964
- The Enzymatic Synthesis of Diaminopimelic AcidJournal of Biological Chemistry, 1958
- Reaction of ninhydrin in acid solution with straight-chain amino acids containing two amino groups and its application to the estimation of α∈-diaminopimelic acidBiochemical Journal, 1957
- A MODIFIED NINHYDRIN REAGENT FOR THE PHOTOMETRIC DETERMINATION OF AMINO ACIDS AND RELATED COMPOUNDSJournal of Biological Chemistry, 1954
- A MODIFIED PHOTOMETRIC NINHYDRIN METHOD FOR THE ANALYSIS OF AMINO AND IMINO ACIDSJournal of Biological Chemistry, 1953
- PHOTOMETRIC ESTIMATION OF PROLINE AND ORNITHINEJournal of Biological Chemistry, 1952