Active chloride transport in the pigmented rabbit conjunctiva
- 1 January 1993
- journal article
- research article
- Published by Informa UK Limited in Current Eye Research
- Vol. 12 (12), 1041-1048
- https://doi.org/10.3109/02713689309033501
Abstract
The present study demonstrates, for the first time, that the excised pigmented rabbit conjunctiva is a tight barrier capable of active Cl- transport. The transepithelial potential difference was 17.7 +/- 0.8 mV (tear-side negative), the short-circuit current was 14.5 +/- 0.7 microA/cm2, and the transconjunctival resistance was 1.3 +/- 0.1 k omega.cm2 for n = 45 tissues. Various inhibitors including ouabain (a Na+/K(+)-ATPase inhibitor), amiloride (a Na+ transport blocker), N-phenylanthranilic acid (a chloride transport inhibitor), bumetanide (an inhibitor of Na(+)-(K+)-Cl- cotransport process), and BaCl2 (a K+ channel blocker) were used on the mucosal and serosal sides of the tissue mounted in Ussing chambers to determine the involvement of the respective ion transport processes in the observed short-circuit current across the conjunctiva. The results suggest that a Cl- conductive pathway is present on the mucosal side of the conjunctiva, whereas Na+/K(+)-ATPase, Na(+)-(K+)-Cl- cotransport process, and K+ conductive pathways are present on its serosal side. Amiloride-sensitive Na(+)-conductive pathways do not appear to be present on either side of the pigmented rabbit conjunctiva.Keywords
This publication has 21 references indexed in Scilit:
- K(+)-H+ exchange, a fundamental cell acidifier in corneal epitheliumAmerican Journal of Physiology-Cell Physiology, 1991
- Lipophilicity influence on conjunctival drug penetration in the pigmented rabbit: A comparison with corneal penetrationCurrent Eye Research, 1991
- Mechanism of chloride uptake in rabbit corneal epitheliumAmerican Journal of Physiology-Cell Physiology, 1989
- Intracellular pH regulation in basal corneal epithelial cells measured in corneal explants: Characterization of exchangeExperimental Eye Research, 1989
- Comparison of conjunctival and corneal surface areas in rabbit and humanCurrent Eye Research, 1988
- Inhibitory effects of chemically-different ‘loop’ diuretics on chloride transport across the bullfrog corneaActa Physiologica Scandinavica, 1986
- Transport processes across the rabbit corneal epithelium: A reviewCurrent Eye Research, 1985
- Intracellular ion activities and Cl-transport mechanisms in bullfrog corneal epitheliumAmerican Journal of Physiology-Cell Physiology, 1983
- Numerical solution of coupled transport equations applied to corneal hydration dynamics.The Journal of Physiology, 1979
- Active transport of chloride in frog corneaAmerican Journal of Physiology-Legacy Content, 1966