DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment.
Open Access
- 1 June 1976
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 69 (3), 732-736
- https://doi.org/10.1083/jcb.69.3.732
Abstract
A method for permeabilizing chinese hamster ovary cells to nucleotides under conditions which allow most cells to remain viable was developed. Permeabilized cells can carry out ATP-dependent, semiconservative synthesis of DNA. The data are consistent with the continuation of DNA synthesis in those cells in S phase at the time of treatment, possibly limited to completion of replicon synthesis without new initiations.Keywords
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