Production of Monospecific Antisera Against Antigenic Components ofMycobacterium bovis(BCG)

Abstract
Monospecific antisera against 4 different antigenic components of BCG were produced by immunization of rabbits with precipitates cut out of gels after crossed immunoelectrophoresis (CIE). It is important to use a semipurified mycobacterial antigenic preparation, or a cross-reactive antigen, to obtain a simpler pattern in CIE so that a clean precipitate, free from contaminating antigens, can be obtained for immunization. The findings with 1 particular antigen are shown, and the use of such antisera is discussed.