Rational attenuation of Corynebacterium pseudotuberculosis: potential cheesy gland vaccine and live delivery vehicle
- 1 July 1992
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 60 (7), 2900-2905
- https://doi.org/10.1128/iai.60.7.2900-2905.1992
Abstract
The phospholipase D (PLD) gene (pld) has been deleted from the Corynebacterium pseudotuberculosis chromosome by using site-specific mutagenesis. Sheep infection trials indicate that the PLD-negative C. pseudotuberculosis strain (Toxminus) is incapable of inducing caseous lymphadentis (cheesy gland) even at doses two logs higher than that at which the wild-type strain produces the disease. This clearly establishes PLD as a major C. pseudotuberculosis virulence factor. Vaccination of sheep with live Toxminus C. pseudotuberculosis elicits strong humoral and cell-mediated immune responses and protects the animals from wild-type challenge.Keywords
This publication has 18 references indexed in Scilit:
- Humoral and cell-mediated immune responses to live recombinant BCG–HIV vaccinesNature, 1991
- New use of BCG for recombinant vaccinesNature, 1991
- Construction and characterization of a Mycobacterium-Escherichia coli shuttle vectorPlasmid, 1991
- Detection of a gene encoding a phosphatidylinositol‐specific phospholipase C that is co‐ordinately expressed with listeriolysin in Listeria monocytogenesMolecular Microbiology, 1991
- Identification of phosphatidylinositol‐specific phospholipase C activity in Listeria monocytogenes: a novel type of virulence factor?Molecular Microbiology, 1991
- A sandwich enzyme immunoassay for bovine interferon‐γ and its use for the detection of tuberculosis in cattleAustralian Veterinary Journal, 1990
- Phospholipase C and haemolytic activities of Clostridium perfringens alpha‐toxin cloned in Escherichia coli: sequence and homology with a Baciilus cereus phospholipase CMolecular Microbiology, 1989
- The effects of caseous lymphadenitis on wool production and bodyweight in young sheepAustralian Veterinary Journal, 1988
- Pathogenesis of caseous lymphadenitis in sheep and goatsAustralian Veterinary Journal, 1986
- Identification and characterization of a new enzyme of the group “phospholipase D” isolated from Corynebacterium ovisBiochimica et Biophysica Acta (BBA) - Enzymology, 1971