Characterization and genomic analysis of kraft lignin biodegradation by the beta-proteobacterium Cupriavidus basilensis B-8
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Open Access
- 8 January 2013
- journal article
- Published by Springer Science and Business Media LLC in Biotechnology for Biofuels
- Vol. 6 (1), 1
- https://doi.org/10.1186/1754-6834-6-1
Abstract
Lignin materials are abundant and among the most important potential sources for biofuel production. Development of an efficient lignin degradation process has considerable potential for the production of a variety of chemicals, including bioethanol. However, lignin degradation using current methods is inefficient. Given their immense environmental adaptability and biochemical versatility, bacterial could be used as a valuable tool for the rapid degradation of lignin. Kraft lignin (KL) is a polymer by-product of the pulp and paper industry resulting from alkaline sulfide treatment of lignocellulose, and it has been widely used for lignin-related studies. Beta-proteobacterium Cupriavidus basilensis B-8 isolated from erosive bamboo slips displayed substantial KL degradation capability. With initial concentrations of 0.5–6 g L-1, at least 31.3% KL could be degraded in 7 days. The maximum degradation rate was 44.4% at the initial concentration of 2 g L-1. The optimum pH and temperature for KL degradation were 7.0 and 30°C, respectively. Manganese peroxidase (MnP) and laccase (Lac) demonstrated their greatest level of activity, 1685.3 U L-1 and 815.6 U L-1, at the third and fourth days, respectively. Many small molecule intermediates were formed during the process of KL degradation, as determined using GC-MS analysis. In order to perform metabolic reconstruction of lignin degradation in this bacterium, a draft genome sequence for C. basilensis B-8 was generated. Genomic analysis focused on the catabolic potential of this bacterium against several lignin-derived compounds. These analyses together with sequence comparisons predicted the existence of three major metabolic pathways: β-ketoadipate, phenol degradation, and gentisate pathways. These results confirmed the capability of C. basilensis B-8 to promote KL degradation. Whole genomic sequencing and systematic analysis of the C. basilensis B-8 genome identified degradation steps and intermediates from this bacterial-mediated KL degradation method. Our findings provide a theoretical basis for research into the mechanisms of lignin degradation as well as a practical basis for biofuel production using lignin materials.Keywords
This publication has 39 references indexed in Scilit:
- De Novo Genome Project of Cupriavidus basilensis OR16Journal of Bacteriology, 2012
- Biodegradation of kraft lignin by a bacterial strain Comamonas sp. B-9 isolated from eroded bamboo slipsJournal of Applied Microbiology, 2012
- Pathways for degradation of lignin in bacteria and fungiNatural Product Reports, 2011
- Bacterial decolorization of black liquor in axenic and mixed condition and characterization of metabolitesBiodegradation, 2010
- Direct Identification of a Bacterial Manganese(II) Oxidase, the Multicopper Oxidase MnxG, from Spores of Several Different Marine Bacillus SpeciesApplied and Environmental Microbiology, 2008
- RNAmmer: consistent and rapid annotation of ribosomal RNA genesNucleic Acids Research, 2007
- Biodegradation of kraft-lignin by Bacillus sp. isolated from sludge of pulp and paper millBiodegradation, 2007
- Identifying bacterial genes and endosymbiont DNA with GlimmerBioinformatics, 2007
- Biodegradation and biological treatments of cellulose, hemicellulose and lignin: an overviewInternational Microbiology, 2002
- Salicylate 5-Hydroxylase from Ralstonia sp. Strain U2: a Monooxygenase with Close Relationships to and Shared Electron Transport Proteins with Naphthalene DioxygenaseJournal of Bacteriology, 2002