Co-transport of Potassium and Sugars across the Plasmalemma of Mesophyll Protoplasts
- 1 January 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 67 (1), 163-169
- https://doi.org/10.1104/pp.67.1.163
Abstract
Sugars (sucrose + hexoses) produced photosynthetically by isolated mesophyll protoplasts of wheat (triticum aestivum) and tobacco (Nicotiana tabacum) were effluxed across the plasma membrane (3-10 .mu.mol hexose equivalents per milligram chlorophyll per hour). The efflux was sensitive to uncouplers and oligomycin which indicated a requirement for energy. A proton gradient was probably not coupled directly to the transport because changing the proton gradient across the plasma membrane by varying the pH of the medium, or by adding sodium acetate, had no significant effect on the rate of sugar release. A release of K+ was associated with sugar efflux from the protoplasts. The molar ratio of K+ to sugar varied between 1.5 and 2.5, depending on the species. Exogenous KCl, RbCl and LiCl (50 mm each), but not NaCl or CsCl, significantly inhibited sugar efflux. Conditions that reduced sugar efflux (exogenous KCl, LiCl, mersalyl, or oligomycin) also reduced K+ release and caused a time-dependent reduction in photosynthetic sucrose formation and increased amino acid and starch formation. Results obtained support the postulate that a K+ symport is involved in the transport of sugar across the energized plasmalemma of photosynthetically active mesophyll cells.This publication has 29 references indexed in Scilit:
- TranslocationPlant Physiology, 1980
- The site of sucrose synthesis in isolated leaf protoplastsFEBS Letters, 1979
- Effect of Exogenously Supplied Foliar Potassium on Phloem Loading in Beta vulgaris L.Plant Physiology, 1979
- Correlation of Adenosine Triphosphate Levels in Chara corallina with the Activity of the Electrogenic PumpPlant Physiology, 1979
- Ion Transport in Isolated Protoplasts from Tobacco Suspension CellsPlant Physiology, 1979
- Evidence for Amino Acid-H+ Co-Transport in Oat ColeoptilesPlant Physiology, 1978
- Use of salicylic acid to measure the apparent intracellular pH in the ehrlich ascites-tumor cell and Escherichia coliBiochimica et Biophysica Acta (BBA) - Biomembranes, 1978
- Intracellular pH transients induced by CO2 or NH3Respiration Physiology, 1978
- The Action of Valinomycin in Uncoupling Corn MitochondriaPlant Physiology, 1975
- Evidence for an electrogenic ion pump in Nitella translucens. I. The effects of pH, K+, Na+, light and temperature on the membrane potential and resistanceBiochimica et Biophysica Acta (BBA) - Biomembranes, 1972