Tetracalcium phosphate composite containing quaternary ammonium dimethacrylate with antibacterial properties
- 21 December 2011
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part B: Applied Biomaterials
- Vol. 100B (3), 726-734
- https://doi.org/10.1002/jbm.b.32505
Abstract
Tooth caries is a carbohydrate-modified bacterial infectious disease, and recurrent caries is a frequent reason for restoration failure. The objective of this study was to develop a novel antibacterial composite using tetracalcium phosphate (TTCP) fillers and bis(2-methacryloyloxy-ethyl) dimethyl-ammonium bromide, which is a quaternary ammonium dimethacrylate (QADM). QADM was synthesized using 2-(N,N-dimethylamino)ethyl methacrylate and 2-bromoethyl methacrylate and incorporated into a resin. The resin was filled with 40% TTCP and 30% glass particles. The following QADM mass fractions in the composite were tested: 0%, 6%, 12%, and 18%. Streptococcus mutans biofilms were formed on the composites and the colony-forming units (CFUs), metabolic activity, and lactic acid production were measured. The TTCP-QADM composite had flexural strength and elastic modulus similar to those of two commercial composites (p > 0.1). Increasing the QADM content in TTCP composite greatly decreased the bacteria growth and biofilm matrix production. There were significantly more dead bacteria with increasing QADM content. TTCP composite containing 18% QADM had biofilm CFU, metabolic activity, and acid production about half of those without QADM. Inversely linear relationships were established between QADM mass fraction and S. mutans biofilm CFU, metabolic activity, and acid production, with correlation coefficients R2 ≥ 0.98. In conclusion, TTCP-QADM composites were developed and the effect of QADM mass fraction on the antibacterial properties of the composite was determined for the first time. The novel TTCP-QADM composites possessing a strong antibacterial capability, together with calcium phosphate ion release and good mechanical properties, are promising for dental restorations to reduce biofilm growth and recurrent caries. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.Keywords
This publication has 54 references indexed in Scilit:
- Antibacterial and physical properties of calcium–phosphate and calcium–fluoride nanocomposites with chlorhexidineDental Materials, 2012
- Synthesis and characterization of dimethacrylates containing quaternary ammonium functionalities for dental applicationsDental Materials, 2012
- The Inhibitory Effects of Quaternary Ammonium Methacrylates on Soluble and Matrix-bound MMPsJournal of Dental Research, 2011
- Recent Advances and Developments in Composite Dental Restorative MaterialsJournal of Dental Research, 2010
- Strong Nanocomposites with Ca, PO4, and F Release for Caries InhibitionJournal of Dental Research, 2009
- Dental glass‐reinforced composite for caries inhibition: Calcium phosphate ion release and mechanical propertiesJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2009
- Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutansJournal of Dentistry, 2009
- In vitro remineralization of enamel by polymeric amorphous calcium phosphate composite: Quantitative microradiographic studyDental Materials, 2009
- Calcium and phosphate ion releasing composite: Effect of pH on release and mechanical propertiesDental Materials, 2008
- Injectable and strong nano-apatite scaffolds for cell/growth factor delivery and bone regenerationDental Materials, 2008