Corticotropin, lipotropin, and beta-endorphin production by a human nonpituitary tumor in culture: evidence for a common precursor.
- 1 October 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 75 (10), 5160-5164
- https://doi.org/10.1073/pnas.75.10.5160
Abstract
A continuous line (DMS-79) of human pulmonary small cell carcinoma cells secretes immunoreactive ACTH, lipotropin and .beta.-endorphin concomitantly into the culture medium. Gel filtration of the culture medium demonstrated at least 5 components: high MW material(s) that had ACTH, lipotropin and .beta.-endorphin immunoreactivities and materials similar to ACTH, .beta.-lipotropin, .gamma.-lipotropin and .beta.-endorphin in their immunoreactivities and apparent MW. The same components were observed when gel filtration was carried out in 6M guanidine.HCl, and the high MW material(s) appeared to consist of more than 1 component, with MW in the range of 15,000-40,000. Immune affinity chromatography of the high MW component(s) from gel filtration with a specific anti-(1-24)ACTH serum demonstrated that the ACTH, lipotropin and .beta.-endorphin immunoreactivities were possessed by the same molecule(s), suggesting that ACTH, lipotropins and .beta.-endorphin were derived from a common MW precursor.This publication has 21 references indexed in Scilit:
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