Aligned chitosan-based nanofibers for enhanced myogenesis
- 1 January 2010
- journal article
- Published by Royal Society of Chemistry (RSC) in Journal of Materials Chemistry
- Vol. 20 (40), 8904-8911
- https://doi.org/10.1039/c0jm01841d
Abstract
Tissue-engineered nanofibrous matrices can potentially serve as an implantable scaffold for the reconstruction of damaged or lost tissue by regulating cell proliferation, organization, and function. In this study, we developed a polyblend chitosan-polycaprolactone (PCL) nanofibrous scaffold with unidirectional fiber orientation by electrospinning for skeletal muscle tissue reconstruction and investigated the effect of the fiber alignment on cell organization and differentiation in comparison with randomly oriented nanofibers and 2D films of the same material. The chitosan-PCL material was shown to support skeletal muscle cell attachment and proliferation, and the fiber alignment promoted skeletal muscle cell morphogenesis and aligned myotube formation in the nanofiber orientation. Reverse-transcription PCR analyses revealed an up-regulation of differentiation-specific genes, troponin T and myosin heavy chain, in muscle cells on the aligned nanofiber scaffolds, confirming the ability of aligned chitosan-PCL nanofibers to enhance muscle cell differentiation. These results suggest that chitosan-PCL nanofibrous scaffolds with unidirectional fiber orientation can significantly enhanced muscle cell development making it a potential scaffold for enhanced skeletal myogenesis.Keywords
This publication has 26 references indexed in Scilit:
- The application of nanofibrous scaffolds in neural tissue engineeringAdvanced Drug Delivery Reviews, 2009
- Collagen matrices from sponge to nano: new perspectives for tissue engineering of skeletal muscleBMC Biotechnology, 2009
- Review Paper: A Review of the Cellular Response on Electrospun Nanofibers for Tissue EngineeringJournal of Biomaterials Applications, 2008
- Advancing Tissue Engineering by Using Electrospun NanofibersRegenerative Medicine, 2008
- The influence of electrospun aligned poly(ɛ-caprolactone)/collagen nanofiber meshes on the formation of self-aligned skeletal muscle myotubesBiomaterials, 2008
- Skeletal myogenesis on highly orientated microfibrous polyesterurethane scaffoldsJournal of Biomedical Materials Research Part A, 2007
- Engineering controllable anisotropy in electrospun biodegradable nanofibrous scaffolds for musculoskeletal tissue engineeringJournal of Biomechanics, 2006
- Nanofiber alignment and direction of mechanical strain affect the ECM production of human ACL fibroblastBiomaterials, 2005
- Electrospun degradable polyesterurethane membranes: potential scaffolds for skeletal muscle tissue engineeringBiomaterials, 2005
- Potential of Nanofiber Matrix as Tissue-Engineering ScaffoldsTissue Engineering, 2005