p120ctn Acts as an Inhibitory Regulator of Cadherin Function in Colon Carcinoma Cells
Open Access
- 3 May 1999
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 145 (3), 551-562
- https://doi.org/10.1083/jcb.145.3.551
Abstract
P120ctn binds to the cytoplasmic domain of cadherins but its role is poorly understood. Colo 205 cells grow as dispersed cells despite their normal expression of E-cadherin and catenins. However, in these cells we can induce typical E-cadherin–dependent aggregation by treatment with staurosporine or trypsin. These treatments concomitantly induce an electrophoretic mobility shift of p120ctn to a faster position. To investigate whether p120ctn plays a role in this cadherin reactivation process, we transfected Colo 205 cells with a series of p120ctn deletion constructs. Notably, expression of NH2-terminally deleted p120ctn induced aggregation. Similar effects were observed when these constructs were introduced into HT-29 cells. When a mutant N-cadherin lacking the p120ctn-binding site was introduced into Colo 205 cells, this molecule also induced cell aggregation, indicating that cadherins can function normally if they do not bind to p120ctn. These findings suggest that in Colo 205 cells, a signaling mechanism exists to modify a biochemical state of p120ctn and the modified p120ctn blocks the cadherin system. The NH2 terminus–deleted p120ctn appears to compete with the endogenous p120ctn to abolish the adhesion-blocking action.Keywords
This publication has 66 references indexed in Scilit:
- The Membrane-proximal Region of the E-Cadherin Cytoplasmic Domain Prevents Dimerization and Negatively Regulates Adhesion ActivityThe Journal of cell biology, 1998
- Tyrosine Phosphorylation and Src Family Kinases Control Keratinocyte Cell–Cell AdhesionThe Journal of cell biology, 1998
- Acquisition of cell adhesion and induction of focal adhesion kinase of human colon cancer Colo 201 cells by retinoic acid-induced differentiationDifferentiation, 1998
- Induction of tyrosine phosphorylation and association of β-catenin with EGF receptor upon tryptic digestion of quiescent cells at confluenceOncogene, 1997
- The Novel Catenin p120casBinds Classical Cadherins and Induces an Unusual Morphological Phenotype in NIH3T3 FibroblastsExperimental Cell Research, 1996
- A Novel Monoclonal Antibody Specific for Sialylated MUC1 MucinJapanese Journal of Cancer Research, 1996
- A mechanism for inhibition of E-cadherin-mediated cell-cell adhesion by the membrane-associated mucin episialin/MUC1.Molecular Biology of the Cell, 1996
- Labeling Neural Cells Using Adenoviral Gene Transfer of Membrane-Targeted GFPNeuron, 1996
- Regulation of embryonic cell adhesion by the cadherin cytoplasmic domainCell, 1992
- Efficient selection for high-expression transfectants with a novel eukaryotic vectorGene, 1991