Differentiation of Listeria monocytogenes Serovars by Using Artificial Neural Network Analysis of Fourier-Transformed Infrared Spectra
- 1 February 2007
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 73 (3), 1036-1040
- https://doi.org/10.1128/aem.02004-06
Abstract
A classification system based on Fourier transform infrared (FTIR) spectroscopy combined with artificial neural network analysis was designed to differentiate 12 serovars of Listeria monocytogenes using a reference database of 106 well-defined strains. External validation was performed using a test set of another 166 L. monocytogenes strains. The O antigens (serogroup) of 164 strains (98.8%) could be identified correctly, and H antigens were correctly determined in 152 (91.6%) of the test strains. Importantly, 40 out of 41 potentially epidemic serovar 4b strains were unambiguously identified. FTIR analysis is superior to PCR-based systems for serovar differentiation and has potential for the rapid, simultaneous identification of both species and serovar of an unknown Listeria isolate by simply measuring a whole-cell infrared spectrum.Keywords
This publication has 35 references indexed in Scilit:
- How the Bacterial PathogenListeria monocytogenesMediates the Switch from Environmental Dr. Jekyll to Pathogenic Mr. HydeInfection and Immunity, 2006
- Occurrence of Listeria spp. in Effluents of French Urban Wastewater Treatment PlantsApplied and Environmental Microbiology, 2005
- Long-Term Survival of Pathogenic and Sanitation Indicator Bacteria in Experimental Biowaste CompostsApplied and Environmental Microbiology, 2005
- Use of Fourier transform infrared spectra of crude bacterial lipopolysaccharides and chemometrics for differentiation of Salmonella enterica serotypesJournal of Applied Microbiology, 2005
- Outbreak of Clinical Listeriosis in Sheep: Evaluation from possible Contamination Routes from Feed to Raw Produce and HumansJournal of Veterinary Medicine, Series B, 2005
- A Novel Serotype-Specific Gene Cassette ( gltA-gltB ) Is Required for Expression of Teichoic Acid-Associated Surface Antigens in Listeria monocytogenes of Serotype 4bJournal of Bacteriology, 2001
- Rapid identification of Streptococcus and Enterococcus species using diffuse reflectance-absorbance Fourier transform infrared spectroscopy and artificial neural networksFEMS Microbiology Letters, 1996
- Elaboration of a procedure for identification of bacteria using Fourier-Transform IR spectral libraries: a stepwise correlation approachJournal of Microbiological Methods, 1991
- Microbiological characterizations by FT-IR spectroscopyNature, 1991
- Classification and identification of bacteria by Fourier-transform infrared spectroscopyMicrobiology, 1991