Development of a species-specific RNA polymerase I-based shRNA expression vector
Open Access
- 7 December 2006
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 35 (2), e10
- https://doi.org/10.1093/nar/gkl1045
Abstract
RNA interference (RNAi) can be induced in vitro either by application of synthetic short interfering RNAs (siRNAs), or by intracellular expression of siRNAs or short hairpin RNAs (shRNAs) from transfected vectors. The most widely used promoters for siRNA/shRNA expression are based on polymerase III (Pol III)-dependent transcription. We developed an alternative vector for siRNA/shRNA expression, using a mouse RNA polymerase I (Pol I) promoter. Pol I-dependent transcription serves in cells for production of ribosomal RNA (rRNA), and as such, is ubiquitously and stably active in different cell types. As Pol I-dependent transcription is highly species-specific, Pol I-based system provides an important biosafety advantage with respect to silencing of genes with unknown functions.Keywords
This publication has 36 references indexed in Scilit:
- RNA silencingTrends in Biochemical Sciences, 2005
- RNAi technology and lentiviral delivery as a powerful tool to suppress Tpr-Met-mediated tumorigenesisCancer Gene Therapy, 2005
- RNA-mediated gene silencing: mechanisms and its therapeutic applicationsJournal of Clinical Pharmacy & Therapeutics, 2004
- Unlocking the potential of the human genome with RNA interferenceNature, 2004
- Effective expression of small interfering RNA in human cellsNature Biotechnology, 2002
- U6 promoter–driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cellsNature Biotechnology, 2002
- A System for Stable Expression of Short Interfering RNAs in Mammalian CellsScience, 2002
- RNAiCell, 2000
- A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in PlantsScience, 1999
- Termination sequence requirements vary among genes transcribed by RNA polymerase III 1 1Edited by J. KarnJournal of Molecular Biology, 1999