Urinary Biomarkers of Oxidative Status in a Clinical Model of Oxidative Assault
Open Access
- 1 June 2010
- journal article
- Published by American Association for Cancer Research (AACR) in Cancer Epidemiology, Biomarkers & Prevention
- Vol. 19 (6), 1506-1510
- https://doi.org/10.1158/1055-9965.epi-10-0211
Abstract
Background: We used doxorubicin-based chemotherapy as a clinical model of oxidative assault in humans. Methods: The study recruited newly diagnosed breast cancer patients (n = 23). Urine samples were collected immediately before (T0) and at 1 hour (T1) and 24 hours (T24) after i.v. administration of treatment. Measurements included allantoin and the isoprostanes iPF(2α)-III, iPF(2α)-VI, and 8,12-iso-iPF(2α)-VI along with the prostaglandin 2,3-dinor-iPF(2α)-III, a metabolite of iPF(2α)-III. All biomarkers were quantified using liquid chromatography–tandem mass spectrometry. Results: In all subjects, the levels of the biomarkers increased at T1: allantoin by 22% (P = 0.06), iPF(2α)-III by 62% (P < 0.05), iPF(2α)-VI by 41% (P < 0.05), 8,12-iso-iPF(2α)-VI by 58% (P < 0.05), and 2,3-dinor-iPF(2α)-III by 52% (P < 0.05). At T24, the F2-isoprostanes returned to their baseline levels; the levels of allantoin continued to increase, although the T24-T0 difference was not statistically significant. Conclusions: These results indicate that urinary F2-isoprostanes are valid biomarkers and allantoin is a promising biomarker of oxidative status in humans. Impact: The levels of biomarkers change quickly in response to oxidative assault and can be used to monitor oxidative status in humans in response to treatments related either to generation of free radicals (chemotherapy and radiation therapy) or to antioxidants (inborn metabolic diseases and Down syndrome). Cancer Epidemiol Biomarkers Prev; 19(6); 1506–10. ©2010 AACR.Keywords
This publication has 16 references indexed in Scilit:
- Using Isoprostanes as Biomarkers of Oxidative Stress: Some Rarely Considered IssuesAntioxidants and Redox Signaling, 2010
- Allantoin in Human Plasma, Serum, and Nasal-Lining Fluids as a Biomarker of Oxidative Stress: Avoiding Artifacts and Establishing Realin vivoConcentrationsAntioxidants and Redox Signaling, 2009
- Markers of oxidative status in a clinical model of oxidative assault: a pilot study in human blood following doxorubicin administrationBiomarkers, 2009
- Quantitation of isoprostane isomers in human urine from smokers and nonsmokers by LC-MS/MSJournal of Lipid Research, 2007
- HPLC–Atmospheric Pressure Chemical Ionization MS/MS for Quantification of 15-F2t-Isoprostane in Human Urine and PlasmaClinical Chemistry, 2007
- Biomarkers of oxidative stress studyFree Radical Biology & Medicine, 2004
- Biomarkers of Oxidative Stress Study II: Are oxidation products of lipids, proteins, and DNA markers of CCl4 poisoning?Free Radical Biology & Medicine, 2004
- Anthracyclines: Molecular Advances and Pharmacologic Developments in Antitumor Activity and CardiotoxicityPublished by American Society for Pharmacology & Experimental Therapeutics (ASPET) ,2004
- Measuring reactive species and oxidative damagein vivoand in cell culture: how should you do it and what do the results mean?British Journal of Pharmacology, 2004
- Uric acid provides an antioxidant defense in humans against oxidant- and radical-caused aging and cancer: a hypothesis.Proceedings of the National Academy of Sciences, 1981