Structural reorganizations in lipid bilayer systems: effect of hydration and sterol addition on Raman spectra of dipalmitoylphosphatidylcholine multilayers
- 16 September 1980
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 19 (19), 4429-4436
- https://doi.org/10.1021/bi00560a008
Abstract
Vibrational Raman spectroscopy was used to investigate the conformational behavior of dipalmitoylphosphatidylcholine (DPPC) bilayers perturbed by cholesterol and water, 2 membrane components whose lipid interactions involve different regions of the bilayer matrix. Upon the addition of cholesterol, an intrinsic membrane constituent, to an anhydrous bilayer in concentrations varying from 7 to 30 mol %, modifications in lateral chain interactions were observed by monitoring spectral changes in the methylene C-H stretching and the CH2 deformation regions. The perturbation in the 1460-cm-1 region was not spectroscopically observed until after the addition of 7 mol % of the sterol. Although chain-chain interactions are altered, no additional trans/gauche isomerization is developed along the hydrocarbon chains. Water, a peripheral bilayer component, was added to the multilayer assembly in the hydration range of 0.3 to .apprx. 4 molecules of water/lipid molecule. Vibrational spectra characteristic of motions in the head-group, interfacial and acyl chain regions of the lipid bilayer were observed. Hydration confers a mobility to the head-group, glycerol and carbonyl moieties. Shifts in the CN symmetric and PO2- antisymmetric stretching modes, occurring on the addition of approximately 4 molecules of water, indicate a conformational rearrangement within the polar head group. After .apprx. 4 water molecules are added to the DPPC system, the spectral features of the gel system [70% (wt/wt) water] indicate that no further head-group changes nor increases in either acyl chain trans/gauche or lattice disorder arise on further hydration.Keywords
This publication has 17 references indexed in Scilit:
- Raman spectra and vibrational assignments for deuterated membrane lipidsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1977
- Hydrocarbon chain disorder in lipid bilayersBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1977
- Studies on the hydrogen belts of membranes: II. Non‐electrolyte permeability of liposomes of diester, diether, and dialkyl phosphatidylcholine and cholesterolLipids, 1977
- Configurations of fatty acyl chains in egg phosphatidylcholine-cholesterol mixed bilayersChemistry and Physics of Lipids, 1977
- Comment on the carbon-hydrogen stretching region of vibrational raman spectra of phospholipidsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1977
- A structural model for the cholesterol‐phosphatidylcholine complexes in bilayer membranesLipids, 1977
- On the quantitative interpretation of biomembrane structure by Raman spectroscopyBiochimica et Biophysica Acta (BBA) - Biomembranes, 1977
- Effect of bilayer curvature on vibrational Raman spectroscopic behavior of phospholipid-water assembliesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- Interactions of the cholesterol side-chain with egg lecithin a spin label studyBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- Factors affecting the motion of the polar headgroup in phospholipid bilayers. A 31P NMR study of unsonicated phosphatidylcholine liposomesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976