A study of the determination of glucuronic acid by the naphthoresorcinol reaction, with the photoelectric absorptiometer

Abstract

The relation of time of heating and of cone, of HC1 and of naphthoresorcinol to color production was studied, the intensity of color in amyl ale. being measured in the Spekker photoelectric absorptiometer with an orange filter. Under the standard conditions described glucuronides were estimated with an accuracy of [plus or minus] 5% in the absence of certain interfering substances such as glucose and fructose. For certain glucuronides, however, the time of heating was increased above the standard conditions adopted for routine estimations. The method was applied to urine and the daily output of glucuronic acid was found to be of the order of 1 g. for adult humans and 140-150 mg. for rabbits. The significance of these values is discussed.