The proton NMR spectrum in acute EAE: The significance of the change in the Cho:Cr ratio
- 1 June 1993
- journal article
- research article
- Published by Wiley in Magnetic Resonance in Medicine
- Vol. 29 (6), 737-745
- https://doi.org/10.1002/mrm.1910290605
Abstract
We have studied by nuclear magnetic resonance spectroscopy the evolution of the proton spectral changes in acute experimental allergic encephalomyelitis. We found an in vivo elevation in the ratio between the peaks assigned to “choline containing compounds” (Cho) and creatine plus phosphocreatine (Cr). This was associated with an increase in choline, betaine, and phosphorylcholine (PC) as well as a reduction in N‐acetylaspartate (NAA), aspartate, N‐acetylaspartatyl‐glutamate and inositol in vitro. Histological examination revealed inflammation with no evidence of demyelination or neuronal loss. We conclude that the increase in the ratio of Cho:Cr was due to an increase in the concentrations of PC, betaine, and choline in association with inflammation, and not as others have suggested, with demyelination. The reported reduction in NAA may be due to dysfunction of neurones rather than their loss.Keywords
This publication has 43 references indexed in Scilit:
- Localized in vivo proton spectroscopy in the brain of patients with multiple sclerosisMagnetic Resonance in Medicine, 1991
- Proton magnetic resonance spectroscopy of an acute and chronic lesion in multiple sclerosisThe Lancet, 1991
- Formation of carbamates of taurine and other amino acids during neutralization of tissue extracts with potassium carbonate/ bicarbonateJournal of Magnetic Resonance (1969), 1990
- Characterization of lipid composition in stimulated human lymphocytes by 1H-NMRBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1990
- Analysis of serial measurements in medical research.BMJ, 1990
- Induction of Ia molecules on brain endothelium is related to susceptibility to experimental allergic encephalomyelitisJournal of Neuroimmunology, 1989
- 1H NMR detection of cerebral myo‐inositolFEBS Letters, 1985
- Betaine, metabolic by-product or vital methylating agent?Life Sciences, 1983
- Chronic relapsing experimental allergic encephalomyelitis: An experimental model of multiple sclerosisAnnals of Neurology, 1977
- CEREBRAL ENERGY STATE IN INSULIN‐INDUCED HYPOGLYCEMIA, RELATED TO BLOOD GLUCOSE AND TO EEGJournal of Neurochemistry, 1974