Identification and Characterization of Thymosin β‐4 in Chicken Macrophages Using Whole Cell MALDI‐TOF
- 1 September 2007
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 1112 (1), 425-434
- https://doi.org/10.1196/annals.1415.028
Abstract
The aim of the study was to determine chicken monocyte- and granulocyte-associated peptides and proteins using "whole cell" matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and to characterize the peptides based on their abundance. The mass spectra showed a prominent peak at m/z 4963 in monocytes/macrophages but not in the granulocytes. Subsequent purification and characterization of the m/z 4963 peptide from an avian macrophage cell line HTC, revealed it to be thymosin beta-4 (Tbeta-4), an actin-modulating peptide. HTC cells when treated with bacterial lipopolysaccharide and peptidoglycan to determine the modulation of Tbeta-4 gene expression or its secretion, showed no changes.Keywords
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