Enzymic sulphation of p-nitrophenol and steroids by larval gut tissues of the southern armyworm (Prodenia eridania Cramer)
- 1 November 1972
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 130 (2), 487-493
- https://doi.org/10.1042/bj1300487
Abstract
1. An enzyme system that catalyses the sulphation of p-nitrophenol, cholesterol, α-ecdysone, β-sitosterol, dehydroepiandrosterone, oestrone and four other steroids of plant and insect origin was obtained from the soluble fraction of southern-armyworm gut tissues. 2. The enzyme system required ATP and inorganic sulphate, and activity was slightly enhanced in the presence of GSH. 3. The properties of this enzyme system with respect to pH, temperature, substrate and protein concentrations and various cofactors and reagents were studied. At −23°C the enzyme preparation could be stored for 2 weeks without drastic loss of activity. At the end of storage for 1 month the loss of activity was approx. 21%. 4. The possible involvement of this enzyme system in insect endocrine control is discussed.Keywords
This publication has 22 references indexed in Scilit:
- Metabolic pathways of steroids in insectsProceedings of the Royal Society of London. B. Biological Sciences, 1972
- Microsomal mixed-function oxidases in insects—I: Localization and properties of an enzyme system effecting aldrin epoxidatton in larvae of the southern armyworm (Prodenia eridania)Biochemical Pharmacology, 1969
- Sterol sulfates in an insectSteroids, 1969
- Formation of serotonin O-sulfate by sulfotransferase of rabbit liverBiochimica et Biophysica Acta (BBA) - General Subjects, 1969
- Kinetic studies of the phenol sulphotransferase reactionBiochimica et Biophysica Acta (BBA) - Enzymology, 1968
- The Comparative Metabolism of XenobioticsPublished by Elsevier BV ,1968
- The formation of cholesteryl sulphate by androstenolone sulphotransferaseBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1967
- Ecdysones from the pupa of the tobacco hornworm, Manduca sexta (Johanson)Steroids, 1966
- Der Ecdysontiter während der InsektenentwicklungDevelopmental Biology, 1965
- Changes in Titer of Ecdysone in Bombyx mori during MetamorphosisScience, 1962