Comparison of the stability of glutathione and related synthetic tetrapeptides by HPLC and capillary electrophoresis
- 1 December 2006
- journal article
- research article
- Published by Wiley in Journal of Peptide Science
- Vol. 12 (12), 796-799
- https://doi.org/10.1002/psc.814
Abstract
Glutathione and related peptides are interesting targets as protectors of biological systems against an oxidative injury. Two novel glutathione analogues, UPF1 and UPF15, have been designed and synthesised. As a result of different reactions taking place, the thiol-containing compounds oxidise to disulfides. In this study, the stability of UPF1, UPF15 and glutathione in various solutions was investigated by using HPLC and CE. The results showed that UPF1 and UPF15 are powerful hydroxyl radical scavengers and their dimerisation process velocity is higher than that of glutathione.Keywords
This publication has 16 references indexed in Scilit:
- Characterization of the antioxidative activity of novel nontoxic neuropeptides by using capillary electrophoresisElectrophoresis, 2006
- Recent developments in capillary electrophoresis and capillary electrochromatography of peptidesElectrophoresis, 2006
- Capillary electrophoresis of glutathione to monitor oxidative stress and response to antioxidant treatments in an animal modelJournal of Chromatography B, 2005
- An antioxidant tetrapeptide UPF1 in rats has a neuroprotective effect in transient global brain ischemiaNeuroscience Letters, 2004
- Age-related changes in glutathione availability and skeletal muscle carbonyl content in healthy ratsExperimental Gerontology, 2004
- Possible Signaling by Glutathione and Its Novel Analogue through Potent Stimulation of Frontocortical G Proteins in Normal Aging and in Alzheimer's DiseaseAnnals of the New York Academy of Sciences, 2002
- Peptidomimetic Glutathione Analogues as Novel γGT Stable GST InhibitorsBioorganic & Medicinal Chemistry, 2002
- Analysis of isomeric glutamyl peptides by capillary electrophoresis: Application to stability studiesJournal of Chromatography A, 2000
- Oxidation of thiocyanate by hydrogen peroxide — a reaction kinetic study by capillary electrophoresisTalanta, 2000
- Determination of reduced and oxidized glutathione in erythrocytes by high-performance liquid chromatography with ultraviolet absorbance detectionJournal of Chromatography B: Biomedical Sciences and Applications, 1996