Prokaryotic 20β‐hydroxysteroid dehydrogenase is an enzyme of the ‘short‐chain, non‐metalloenzyme’ alcohol dehydrogenase type
- 18 June 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 266 (1-2), 51-54
- https://doi.org/10.1016/0014-5793(90)81504-h
Abstract
The primary structure of 20β-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was determined after FPLC purification of a commercial preparation. Peptides obtained from different proteolytic cleavages were purified by reverse phase HPLC. The 255-residue structure deduced was found to be distantly homologous to those of Drosophila alcohol dehydrogenase and several other dehydrogenases, establishing that prokaryotic 20β-hydroxysteroid dehydrogenase as a member of the ‘short-chain alcohol dehydrogenase family’. With the enzymes characterized, the identity is greatest (31–34%) towards 4 other prokaryotic dehydrogenases, but the family also includes mammalian steroid and prostaglandin dehydrogenases. These enzymes are low in Cys and have a strictly conserved Tyr residue that appears to be important.Keywords
This publication has 22 references indexed in Scilit:
- Site-directed mutagenesis of glycine-14 and two "critical" cysteinyl residues in Drosophila alcohol dehydrogenaseBiochemistry, 1990
- Purification and structural characterization of placental NAD+-linked 15-hydroxyprostaglandin dehydrogenase. The primary structure reveals the enzyme to belong to the short-chain alcohol dehydrogenase familyBiochemistry, 1990
- Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolorGene, 1988
- Complete amino acid sequence of human placental 17β‐hydroxysteroid dehydrogenase deduced from cDNAFEBS Letters, 1988
- Class III human liver alcohol dehydrogenase: a novel structural type equidistantly related to the class I and class II enzymesBiochemistry, 1988
- Extended superfamily of short alcohol‐polyol‐sugar dehydrogenases: structural similarities between glucose and ribitol dehydrogenasesFEBS Letters, 1984
- Complete amino acid sequence of glucose dehydrogenase from Bacillus megateriumFEBS Letters, 1984
- Bifunctional enzyme activity at the same active site: study of 3.alpha. and 20.beta. activity by affinity alkylation of 3.alpha.,20.beta.-hydroxysteroid dehydrogenase with 17-(bromoacetoxy)steroidsBiochemistry, 1980
- Structural Analyses of Mutant and Wild-Type Alcohol Dehydrogenases from Drosophila melanogasterEuropean Journal of Biochemistry, 1976