Passaged Adult Chondrocytes Can Form Engineered Cartilage with Functional Mechanical Properties: A Canine Model
- 1 March 2010
- journal article
- research article
- Published by Mary Ann Liebert Inc in Tissue Engineering, Part A
- Vol. 16 (3), 1041-1051
- https://doi.org/10.1089/ten.tea.2009.0581
Abstract
It was hypothesized that previously optimized serum-free culture conditions for juvenile bovine chondrocytes could be adapted to generate engineered cartilage with physiologic mechanical properties in a preclinical, adult canine model. Primary or passaged (using growth factors) adult chondrocytes from three adult dogs were encapsulated in agarose, and cultured in serum-free media with transforming growth factor-β3. After 28 days in culture, engineered cartilage formed by primary chondrocytes exhibited only small increases in glycosaminoglycan content. However, all passaged chondrocytes on day 28 elaborated a cartilage matrix with compressive properties and glycosaminoglycan content in the range of native adult canine cartilage values. A preliminary biocompatibility study utilizing chondral and osteochondral constructs showed no gross or histological signs of rejection, with all implanted constructs showing excellent integration with surrounding cartilage and subchondral bone. This study demonstrates that adult canine chondrocytes can form a mechanically functional, biocompatible engineered cartilage tissue under optimized culture conditions. The encouraging findings of this work highlight the potential for tissue engineering strategies using adult chondrocytes in the clinical treatment of cartilage defects.Keywords
This publication has 53 references indexed in Scilit:
- Transient Exposure to Transforming Growth Factor Beta 3 Under Serum-Free Conditions Enhances the Biomechanical and Biochemical Maturation of Tissue-Engineered CartilageTissue Engineering, Part A, 2008
- The effect of devitalized trabecular bone on the formation of osteochondral tissue-engineered constructsBiomaterials, 2008
- Autogenous Osteochondral Grafting for Treatment of Stifle Osteochondrosis in DogsVeterinary Surgery, 2008
- Amino acids supply in culture media is not a limiting factor in the matrix synthesis of engineered cartilage tissueAmino Acids, 2007
- Growth Factors for Clinical-Scale Expansion of Human Articular Chondrocytes: Relevance for Automated Bioreactor SystemsTissue Engineering, 2007
- The beneficial effect of delayed compressive loading on tissue-engineered cartilage constructs cultured with TGF-β3Osteoarthritis and Cartilage, 2007
- Short‐term outcome after total elbow arthroplasty in dogs with severe, naturally occurring osteoarthritisVeterinary Surgery, 2003
- Canine cemented total hip replacements: State of the artJournal of Small Animal Practice, 1995
- In vitro contact stress distribution on the femoral condylesJournal of Orthopaedic Research, 1984
- A Direct Spectrophotometric Microassay for Sulfated Glycosaminoglycans in Cartilage CulturesConnective Tissue Research, 1982