Lactate reduces glutamate‐induced neurotoxicity in rat cortex
- 30 November 2001
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 66 (5), 790-794
- https://doi.org/10.1002/jnr.10043
Abstract
Experiments were carried out to test the hypothesis that lactate reduces the neurotoxicity of glutamate in vivo. MAP2 immunohistochemistry was used to measure lesion size, and microdialysis to measure the changes in glucose and lactate in the extracellular compartment. After implantation of a microdialysis probe 100 mM glutamate with or without 6 mM lactate was added to the perfusion medium and infused into the cortex of unanesthetized rats. Infusion of 100 mM glutamate for a period of 30 min produced a lesion of 6.05 ± 0.64 mm3, an increase in lactate of 124 ± 19% above basal and a 21 ± 9% reduction of glucose below basal level. When 6mM L‐lactate was perfused together with 100 mM glutamate there was a significant reduction in the size of the lesion and there was no reduction in dialysate glucose. When L‐lactate was replaced with D‐lactate the lesion size and the increase in dialysate lactate were greater than after glutamate alone. The neuroprotective role of L‐lactate is attributed to its ability to meet the increased energy demands of neurones exposed to high concentrations of glutamate.Keywords
This publication has 13 references indexed in Scilit:
- Cellular mechanisms of brain energy metabolism and their relevance to functional brain imagingPhilosophical Transactions Of The Royal Society B-Biological Sciences, 1999
- An Increase in Lactate Output by Brain Tissue Serves to Meet the Energy Needs of Glutamate-Activated NeuronsJournal of Neuroscience, 1999
- Partitioning of CO2 Production Between Glucose and Lactate in Excised Sympathetic Ganglia, with Implications for BrainJournal of Neurochemistry, 1996
- Microtubule-associated protein 2 (MAP-2): a sensitive marker of seizure-related brain damageJournal of Neuroscience Methods, 1995
- Neuroprotective Effects of Glutamate Antagonists and Extracellular AcidityScience, 1993
- 5-(N-ethyl-N-isopropyl)amiloride and mild acidosis protect cultured cerebellar granule cells against glutamate-induced delayed neuronal deathNeuroscience, 1992
- Changes in Cortical Extracellular Levels of Energy-Related Metabolites and Amino Acids following Concussive Brain Injury in RatsJournal of Cerebral Blood Flow & Metabolism, 1990
- The Role of Excitatory Amino Acids and NMDA Receptors in Traumatic Brain InjuryScience, 1989
- Ionic dependence of glutamate neurotoxicityJournal of Neuroscience, 1987
- Guidelines on the recognition of pain, distress and discomfort in experimental animals and an hypothesis for assessmentVeterinary Record, 1985