Auxin Biogenesis
- 1 June 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 57 (6), 850-854
- https://doi.org/10.1104/pp.57.6.850
Abstract
Subcellular fractionation of cucumber (Cucumis sativus L.) seedlings was achieved, and 2 of the enzymes in the auxin biosynthetic pathway were localized. NADH-specific indoleacetaldehyde reductase activity was observed only in the cytosol fractions obtained from separated hypocotyl and cotyledon tissue. In contrast, a portion of the NADPH-specific indoleacetaldehyde reductase activity was associated with a microsomal fraction derived from these tissues. The NADPH-specific indoleacetaldehyde reductase was more firmly associated with the microsomal fraction derived from hypocotyls than with that from the cotyledons. These resutls indicate a division of the terminal steps of auxin biogenesis into at least 2 subcellular compartments.This publication has 14 references indexed in Scilit:
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