The Effects of Actinomycin D, Puromycin, Cycloheximide and Hydroxyurea on 3′,5,3-Triiodo-L-thyronine Stimulated 2-Deoxy-D-glucose Uptake in Chick Embryo Heart Cellsin Vitro*†

Abstract

The uptake of 2-deoxy-D-1-3H-glucose (1-3H-DOG) by cultured chick embryo heart cells was stimulated by L-triiodothyronine (T3). This stimulation began with the exposure to T3, rose linearly for about 8 h and reached a plateau around 24 h. Actinomycin D, puromycin and cycloheximide had no effect on the stimulated sugar uptake during the first 6 h. These agents prevented the further increase in the rate of 1-3H-DOG uptake in T3-treated cultures that occurred between 6-24 h. In the presence of hydroxyurea the uptake of 1-3H-DOG in both control and T3-treated cells was unchanged. Some of the effects of T3 may not require new synthesis of either RNA, proteins or DNA.