Overproduction of cellular and activated Ha-ras proteins by mutating a synthetic gene.
- 1 January 1987
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 35 (12), 4878-4882
- https://doi.org/10.1248/cpb.35.4878
Abstract
A synthetic gene for c-Ha-ras (Val-12) has been modified by cassette mutagenesis using restriction sites, ClaI-BssHII, to encode c-Ha-ras Gly-12. Genes for c-Ha-ras (Leu-61 and Arg-61) have been synthesized by joining newly synthesized oligodeoxyribonucleotides containing appropriate codons, together with previously obtained synthetic fragments. These genes have been expressed in E. coli and the products (p21) isolated. The guanosine diphosphate binding properties and guanosine triphosphatase activities of these p21 derivatives were studied.Keywords
This publication has 7 references indexed in Scilit:
- Biological and biochemical properties of human rasH genes mutated at codon 61Cell, 1986
- Synthesis of a gene for human growth hormone and its expression in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America, 1984
- Intrinsic GTPase activity distinguishes normal and oncogenic ras p21 molecules.Proceedings of the National Academy of Sciences, 1984
- Photoaffinity labeling with GTP of viral p21 ras protein expressed in Escherichia coliJournal of Virology, 1984
- Human genome contains four genes homologous to transforming genes of Harvey and Kirsten murine sarcoma viruses.Proceedings of the National Academy of Sciences, 1982
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977