Objective To investigate the effect of all-out cycling test duration on indices of power, anaerobic lactic energy metabolism, perceived exertion and mood. Methods Nine physically active men undertook four all-out cycling tests of 5, 15, 30 or 45 s from seated stationary start on an ergometer fit with power cranks. The participants completed a Profile of Mood States questionnaire before each test and indicated perceived exertion immediately post-test (Borg 6–20 scale). Indices of anaerobic lactic metabolism were determined from blood lactate concentrations. Results Pacing strategy was apparent in the 45-s tests with lower peak (p0.05). Conclusions An all-out pacing strategy was apparent for at least up to 15 s, with indicators of dampened power in both 30 and 45 s sprints. Reduced power at the start of all-out long-duration sprints support a central control of at least initial pacing strategy.