An efficient method of selecting photoautotrophic cells from cultured heterogeneous cells

Abstract

A new and efficient method was demonstrated for the establishment of photoautotrophic cultures of plant cells. Leaf segments of Atropa belladonna, Datura stramonium and Hyoscyamus niger were inoculated on sugar-free Linsmaier-Skoog agar medium then aerated with 1% CO₂ enriched air under 3,000 to 5,000 lux of illumination. Under these regulated conditions we could select photoautotrophic green cells efficiently, and these cells subsequently have grown well under photoautotrophic conditions.