Occurrence of Trenbolone Acetate Metabolites in Simulated Confined Animal Feeding Operation (CAFO) Runoff
- 12 March 2012
- journal article
- Published by American Chemical Society (ACS) in Environmental Science & Technology
- Vol. 46 (7), 3803-3810
- https://doi.org/10.1021/es204529v
Abstract
Metabolites of androgenic synthetic growth promoters used at confined animal feeding operations (CAFOs) pose a demonstrated ecological risk. To evaluate the transport of trenbolone acetate (TBA) metabolites from beef cattle CAFOs, rainfall simulation experiments were conducted at the University of California, Davis, research CAFO. Steroid concentrations in solid and aqueous samples from the research CAFO and solids samples from a commercial CAFO were analyzed by gas chromatography-tandem mass spectrometry. The data indicate that 17α-trenbolone (17α-TBOH), 17β-trenbolone (17β-TBOH), and trendione (TBO), the three primary TBA metabolites, occur in soils and runoff. Soils at the research CAFO contained up to 8.2 (±1.1) ng/g-dw of 17α-TBOH and 1.2 (±0.1) ng/g-dw of 17β-TBOH, with slightly higher (∼20 ng/g-dw) 17α-TBOH concentrations observed in commercial CAFO soils. In simulated runoff, 17α-TBOH concentrations of 1–350 ng/L and TBO concentrations from 1–170 ng/L were observed. The metabolite 17β-TBOH intermittently occurred in runoff samples at 5–26 ng/L and may be correlated to anaerobic soils. Metabolite concentrations observed in CAFO runoff correspond to 5–15% of potential maximum steroid concentrations predicted by mass balances. First order transformation rates of 0.028/day (25 day half-life) were estimated for 17α-TBOH in CAFO soils. Results suggest that ecologically relevant concentrations of TBA metabolites can be mobilized from CAFO surfaces in storm runoff and may lead to receiving water concentrations at or above ecological effects thresholds for a very limited number of discharge scenarios.Keywords
This publication has 27 references indexed in Scilit:
- Effect of Growth Promotants on the Occurrence of Endogenous and Synthetic Steroid Hormones on Feedlot Soils and in Runoff from Beef Cattle Feeding OperationsEnvironmental Science & Technology, 2012
- Stereoselective Sorption by Agricultural Soils and Liquid−Liquid Partitioning of Trenbolone (17α and 17β) and TrendioneEnvironmental Science & Technology, 2009
- Population Consequences of Environmental Sex ReversalConservation Biology, 2009
- Effect of parental exposure to trenbolone and the brominated flame retardant BDE-47 on fertility in rainbow trout (Oncorhynchus mykiss)Marine Environmental Research, 2008
- Degradation of Synthetic Androgens 17α- and 17β-Trenbolone and Trendione in Agricultural SoilsEnvironmental Science & Technology, 2008
- Collapse of a fish population after exposure to a synthetic estrogenProceedings of the National Academy of Sciences, 2007
- Mobility of the growth promoters trenbolone and melengestrol acetate in agricultural soil: column studiesScience of The Total Environment, 2004
- Sex hormones originating from different livestock production systems: fate and potential disrupting activity in the environmentAnalytica Chimica Acta, 2002
- Widespread Sexual Disruption in Wild FishEnvironmental Science & Technology, 1998
- A Solenoid‐Operated, Variable Intensity Rainfall SimulatorSoil Science Society of America Journal, 1987