Phytochrome Immunoaffinity Purification

Abstract

A phytochrome immunoaffinity purification procedure was developed that yields undegraded oat (Avena sativa L., cv. Garry) phytochrome of > 98% purity within 2 h when starting with a brushite-purified preparation. Immunoaffinity-purified phytochrome, except for its greater purity, is indistinguishable from conventionally purified phytochrome by gel exclusion chromatography, isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The immunoaffinity technique was also used to purify phytochrome from crude oat extracts, from brushite-purified pea (Pisum sativum L., cv. Alaska) and rye (Secale cereale L., cv. Balbo) preparations.