Separation, Concentration, and Transfusion of Platelets.

Abstract

(1) A simple method is presented for the efficient separation and concentration of platelets from human, dog, and guinea pig blood in quantities sufficient for transfusion. (2) This method depends upon the use of Sequesterene Na₂ as an anticoagulant, which prevents the development of platelet stickiness and clumping in shed blood. (3) Platelets prepared by this method and transfused into the thrombopenic, irradiated dog remain in the circulation, reverse the coagulation defect, and prevent hemorrhage.