Widefield Microscopy Images of Tissue Sections by Computer Imaging Techniques

Abstract

A fine photomicrograph covering one whole specimen is very useful in the study of skin histopathology. However, because it is almost impossible to take such a picture with a conventional photomicroscope, we attempted to make one with the aid of a computer. The large field was divided into small fields, which were individually recorded through a photomicroscope. The images were then digitized and processed with a computer to reconstruct the largefield image. A fine seamless image was reconstructed with this method. We can thus extend the field of a photomicroscope with the aid of computer imaging techniques, without impairing the quality. (J Histochem Cytochem 45:461–466, 1997)