An oligonucleotide-based label-free fluorescent sensor: highly sensitive and selective detection of Hg2+ in aqueous samples
- 1 January 2012
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Analytical Methods
- Vol. 4 (5), 1310-1314
- https://doi.org/10.1039/c2ay05922c
Abstract
A highly sensitive and selective detection method for mercury(II) in aqueous samples was developed based on the use of an oligonucleotide-based label-free fluorescent probe. The novel design was composed of two oligonucleotides with partially complementary sequences and the label-free dye ethidium bromide (EB). The fluorescence signal was very low when EB was free in water solution. However, the fluorescence signal increased by nearly 15 times when the DNA duplex was present. This dsDNA–EB system was used as a fluorescent sensor for Hg2+. The presence of Hg2+ led to the release of EB from the grooves of dsDNA and the fluorescence intensity was quenched. In this investigation, the optimal concentrations of dsDNA and EB were 0.285 μM and 0.9 μM, respectively. The calibration curve was linear in the range of 30 to 120 nM for Hg2+ (R2 = 0.992), with a detection limit of 9.5 nM. The method was simple and convenient, and this sensor also provided potential applications for developing aptasensors.This publication has 46 references indexed in Scilit:
- Tools and Tactics for the Optical Detection of Mercuric IonChemical Reviews, 2008
- Dose-dependent inorganic mercury absorption by isolated perfused intestine of rainbow trout, Oncorhynchus mykiss, involves both amiloride-sensitive and energy-dependent pathwaysAquatic Toxicology, 2005
- Sources and remediation for mercury contamination in aquatic systems—a literature reviewEnvironmental Pollution, 2004
- Speciation analysis of mercury in seafood by using high-performance liquid chromatography on-line coupled with cold-vapor atomic fluorescence spectrometry via a post column microwave digestionAnalytica Chimica Acta, 2003
- Review: Environmental exposure to mercury and its toxicopathologic implications for public healthEnvironmental Toxicology and Water Quality, 2003
- Determination of mercury in environmental and biological samples using pyrolysis atomic absorption spectrometry with gold amalgamationAnalytica Chimica Acta, 1999
- Determination of mercury in saliva with a flow-injection systemAnalytica Chimica Acta, 1996
- Determination of total mercury in waters and urine by flow injection atomic absorption spectrometry procedures involving on- and off-line oxidation of organomercury speciesAnalytical Chemistry, 1993
- Metal toxicity in the central nervous system.Environmental Health Perspectives, 1987
- Fossil Fuels as a Source of Mercury PollutionScience, 1971