Release of leukotriene C4 from human polymorphonuclear leucocytes as determined by radioimmunoassay
- 6 September 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 146 (1), 111-114
- https://doi.org/10.1016/0014-5793(82)80715-1
Abstract
Rabbits were immunized with a conjugate of leukotriene (LT) C4 and bovine serum albumin prepared by coupling the single free amino group of the hapten to the protein using gluteraldehyde. Binding of [3H]LTC4 to the antibodies obtained is inhibited by 50% with 1.5 ng LTC4. The relative cross-reaction of LTD4 is 16% and of LTC4-methyl ester 3.6%. The validity of the radioimmunoassay was demonstrated by comparison with bioassay using the isolated guinea pig ileum. Using the radioimmunoassay it could be shown that endogenous LTC4 is released in a dose-dependent manner by human polymorphonuclear leucocytes stimulated with the divalent cation ionophore A23187.Keywords
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