Attempts at Purification of a Murine Strain of Human Poliomyelitis Virus

Abstract

Infected mouse brains were ground with saline, glycine added, clarified by centrifugation, supernatant removed and shaken with ether. After fat removal by centrifuging, acid-Na acetate buffer was added and precipitate centrifuged out and washed in saline-acetate buffer, recentrifuged and resuspended in Na2HPO4. Insoluble precipitate was discarded and supernatant clarified with saturated (NH4)2SO4 soln. The (NH4)2SO4 was dialyzed out of the supernatant. This process concentrates the virus 10-fold and removes 95% of the non-active material. Attempts at chemical extraction of virus from this soln. by chemical means were abandoned because of inactivation of the virus. More satisfactory extraction was obtained by ultracentrifugation.