Cells with regulatory function of the innate and adaptive immune system in primary Sjögren’s syndrome
Open Access
- 29 April 2009
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical and Experimental Immunology
- Vol. 157 (3), 343-349
- https://doi.org/10.1111/j.1365-2249.2009.03966.x
Abstract
The aim of the present study was to describe subsets of cells with regulatory properties in primary Sjögren's syndrome (pSS), and to correlate these cell populations with clinical symptoms. Among the 32 investigated patients, 23 had extraglandular manifestations (EGMs), while nine had only glandular symptoms. Twenty healthy individuals served as controls. The percentages of natural killer (NK), natural killer T cells (NK T), interleukin (IL)-10 producing T regulatory type 1 (Tr1) cells and CD4+CD25+ regulatory T cells (Treg) cells were determined by flow cytometry and serum cytokine levels of IL-4, IL-6, IL-10, tumour necrosis factor (TNF)-α and interferon (IFN)-γ were evaluated by enzyme-linked immunosorbent assay (ELISA). Functional tests were carried out to assess the suppressor properties of Treg cells in patients and controls. Peripheral NK, NK T and Tr1 cell percentages were elevated in pSS, while CD4+CD25+ Treg cells showed reduced frequencies in patients compared to controls. In pSS, elevated percentages of NK T, Tr1 and CD4+CD25+ Treg cells were observed in patients with EGMs, when compared to patients with sicca symptoms only. CD4+CD25+ Treg cell percentages showed a negative correlation with sialometry values. The in vitro functional assay demonstrated lower suppression activity of CD4+CD25+ Treg cells in patients compared to controls. Serum IL-6 and TNF-α levels were elevated, while IL-10 was decreased in patients compared to controls. Negative correlation was found between IL-10 levels and the percentages of Tr1 cells. Changes in the investigated subsets of regulatory cells in pSS may contribute to the development and progression of the disease.Keywords
This publication has 36 references indexed in Scilit:
- Foxp3+ T-Regulatory Cells in Sjögren's Syndrome: Correlation with the Grade of the Autoimmune Lesion and Certain Adverse Prognostic FactorsThe American Journal of Pathology, 2008
- Decreased CD4+CD25+bright T cells in peripheral blood of patients with primary Sjögren's syndromeLupus, 2008
- Myelin-specific regulatory T cells accumulate in the CNS but fail to control autoimmune inflammationNature Medicine, 2007
- Natural killer T cells in families of patients with systemic lupus erythematosus: Their possible role in regulation of IGG productionArthritis & Rheumatism, 2007
- Reciprocal developmental pathways for the generation of pathogenic effector TH17 and regulatory T cellsNature, 2006
- Regulatory T cells in peripheral blood of patients with mixed connective tissue diseaseScandinavian Journal of Rheumatology, 2006
- Classification criteria for Sjogren's syndrome: a revised version of the European criteria proposed by the American-European Consensus GroupAnnals Of The Rheumatic Diseases, 2002
- The thymus and central tolerancePhilosophical Transactions Of The Royal Society B-Biological Sciences, 2001
- Longitudinal study of interleukin-10, tumor necrosis factor- α, anti-U1-snRNP antibody levels and disease activity in patients with mixed connective tissue diseaseScandinavian Journal of Rheumatology, 2001
- Natural killer (NK) T cells are significantly decreased in the peripheral blood of patients with rheumatoid arthritis (RA)Clinical and Experimental Immunology, 1999