Synthesis of aerobactin and a 76,000-dalton iron-regulated outer membrane protein by Escherichia coli K-12-Shigella flexneri hybrids and by enteroinvasive strains of Escherichia coli
- 1 July 1985
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 49 (1), 67-71
- https://doi.org/10.1128/iai.49.1.67-71.1985
Abstract
One of the chromosomal segments associated with the virulence of S. flexneri and transferred to E. coli K-12 by conjugation has been shown to code for the production of aerobactin and for the synthesis of an iron-regulated 76,000-dalton (76K) outer membrane protein. Analysis of various E. coli K-12-S. flexneri transconjugants showed that the genes involved with the synthesis of aerobactin and with the production of the 76K protein were linked to the mtl region of the S. flexneri chromosome. S. flexneri itself synthesized a 76K protein in its outer membrane under iron restriction as well as traces of 81K and 74K proteins. An examination of 4 enteroinvasive strains of E. coli showed that each produced aerobactin and a 76K outer membrane protein during iron-restricted growth. The profile of the iron-regulated proteins expressed by the enteroinvasive strains of E. coli was virtually identical to that expressed by the laboratory-constructed E. coli K-12-S. flexneri hybrids under the same growth conditions.This publication has 21 references indexed in Scilit:
- Antigenic and Molecular Homology of the Ferric Enterobactin Receptor Protein of Escherichia coliMicrobiology, 1985
- Role of Iron in Microbe-Host InteractionsClinical Infectious Diseases, 1983
- Invasive Enteric PathogensClinical Infectious Diseases, 1983
- Microbial Envelope Proteins Related to IronAnnual Review of Microbiology, 1982
- The cloacin receptor of ColV-bearing Escherichia coli is part of the Fe3+-aerobactin transport system.1982
- An inducible outer membrane protein involved in aerobactin-mediated iron transport by Co1V strains ofEscherichia coliFEBS Letters, 1982
- Microbial Iron CompoundsAnnual Review of Biochemistry, 1981
- ColV Plasmid-Mediated, Colicin V-Independent Iron Uptake System of Invasive Strains of Escherichia coliInfection and Immunity, 1980
- Alterations in tRNAs Containing 2‐Methylthio‐N6‐(Δ2‐isopentenyl)‐adenosine during Growth of Enteropathogenic Escherichia coli in the Presence of Iron‐Binding ProteinsEuropean Journal of Biochemistry, 1978
- THYMIDINE-REQUIRING MUTANTS OF PHAGE T4Proceedings of the National Academy of Sciences, 1963