HYDROLYSIS OF CONJUGATED SULFATES OF ESTROGENS BY COMMERCIAL ENZYME PREPARATION OF ASPERGILLUS ORYZAE

Abstract

My-lase P, a commercial enzyme prepn. from A. oryzae, hydrolyzes the conjugated estrogens present in the urine of pregnant mares. After 24 hrs. digestion at pH 6 and 50[degree]C under a layer of toluene with relatively large amts. of Mylase P, about 20% higher yields of estrogens were obtained than after acid hydrolysis. The phenolic fraction isolated after acid hydrolysis contained 4 times as much non-estrogenic material than that isolated after enzyme hydrolysis.