THE EFFECT OF INSULIN UPON THE LEVEL OF BLOOD AMINO ACIDS IN THE EVISCERATED RAT AS RELATED TO THE LEVEL OF BLOOD GLUCOSE

Abstract

Eviscerated c? rats were given continuous intraven. infusions of different concns. of glucose and insulin over a period of 24 hrs. In rats not given insulin, the amino acid concn. of whole blood increased from a normal avg. of 9.9 mg. % to 28.4 mg. % at the end of 24 hrs. The admn. of optimal amts. of insulin completely suppressed this rise. The quantitative effect of insulin in suppressing the rise of blood amino acids was proportional to the dose and was not detd. by the glucose load or by the level of blood glucose. The rate of rise in blood amino acids in rats not given glucose or insulin was steady during the 24-hr. period and was not changed significantly by the intraven. admn. of fluids during a period of 8 hrs. The rate of rise in blood amino acids for 6 hrs. was increased only slightly by a 24-hr. fast and was practically identical to that of similar animals subjected to "functional" evisceration instead of complete evisceration.