Purification of an acid α-glucosidase by dextran-gel filtration
- 1 October 1967
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 105 (1), 35-38
- https://doi.org/10.1042/bj1050035
Abstract
The behavior of rat liver [alpha]-glucosidases on dextran gel (Sephadex G-100) columns was studied. A "retardation" of an acid [alpha]-glucosidase activity was observed. This activity was identified as lysosome [alpha]-(l[long dash])4)-glucosidase. A single gel-filtration step resulted in a 700-fold purification of the enzyme. The same technique was also used to purify the acid [alpha]-glucosidase of human kidney. The acid [alpha]-glucosidases of both tissues show very similar pH optima when tested with maltose or glycogen as substrate.This publication has 10 references indexed in Scilit:
- Dextran-gel filtration of enzymes in the presence of their high-molecular-weight substratesBiochemical Journal, 1966
- The subcellular distribution of enzymes in type II glycogenosis and the occurrence of an oligo-α-1,4-glucan glucohydrolase in human tissuesBiochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 1965
- Estimation of the molecular weights of proteins by Sephadex gel-filtrationBiochemical Journal, 1964
- LIVER ALPHA-GLUCOSIDASES1964
- BESTIMMUNG DES MOLEKULARGEWICHTS VON ENZYMEN BEI DEXTRANGELFILTRATION1964
- ZUM MOLEKULARGEWICHT DER LACTAT-DEHYDROGENASEN - STUDIEN DES VERHALTENS BEI DER GELFILTRATION1963
- α-Glucosidase deficiency in generalized glycogen-storage disease (Pompe's disease)Biochemical Journal, 1963
- Determination of maltase and isomaltase activities with a glucose-oxidase reagentBiochemical Journal, 1961
- Vergleichende Untersuchungen zur quantitativen Eiweissbestimmung im Liquor und eiweissarmen L sungenKlinische Wochenschrift, 1955
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951