Carbohydrate Composition of Hepatitis B Surface Antigen

Abstract
The content and composition of carbohydrate in hepatitis B surface antigen HB8Ag) were clarified by gas chromatography. A value of 75.8 µg carbohydrate per mg of protein was obtained. The main components were N-acetylglucosamine, mannose, galactose and sialic acid and the minor one was fucose. No N-acetyl-galactosamine was detected. The fact that no sugar was detected in lipid fractions suggests that the sugar in HB8Ag exists almost exclusively in the form of glycoprotein and there is no glycolipid.