The Flocculation Test and the Law of Mass Action
- 31 March 1965
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 94 (4), 586-591
- https://doi.org/10.4049/jimmunol.94.4.586
Abstract
Experiments are presented which show that flocculation time in both the Ramon and Dean-Webb methods is the resultant of two different interacting variables which affect the velocity of precipitation. One is the independent variable of interest, namely, the equivalence ratio. The other is the mass-law effect, that is, the variation from tube to tube of the product of the concentrations of the reactants which is a necessary consequence of keeping one reactant constant. A new, equally simple method is proposed which eliminates the mass-law effect by maintaining a constant product of concentrations. Comparisons of all three methods are presented in both the diphtheria and tetanus systems.Keywords
This publication has 4 references indexed in Scilit:
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- THE ESSENTIAL DIFFERENCE BETWEEN THE TWO OPTIMUM PROPORTIONS FLOCCULATION RATIOSThe Journal of Experimental Medicine, 1944
- INFLUENCE OF CHARACTER OF ANTIBODY UPON VELOCITY OF FLOCCULATIONThe Journal of Experimental Medicine, 1941
- The influence of optimal proportions of antigen and antibody in the serum precipitation reactionThe Journal of Pathology and Bacteriology, 1926