Over-Expression of VvWRKY1 in Grapevines Induces Expression of Jasmonic Acid Pathway-Related Genes and Confers Higher Tolerance to the Downy Mildew
Open Access
- 14 January 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (1), e54185
- https://doi.org/10.1371/journal.pone.0054185
Abstract
Most WRKY transcription factors activate expression of defence genes in a salicylic acid- and/or jasmonic acid-dependent signalling pathway. We previously identified a WRKY gene, VvWRKY1, which is able to enhance tolerance to fungal pathogens when it is overexpressed in tobacco. The present work analyzes the effects of VvWRKY1 overexpression in grapevine. Microarray analysis showed that genes encoding defence-related proteins were up-regulated in the leaves of transgenic 35S::VvWRKY1 grapevines. Quantitative RT-PCR analysis confirmed that three genes putatively involved in jasmonic acid signalling pathway were overexpressed in the transgenic grapes. The ability of VvWRKY1 to trans-activate the promoters of these genes was demonstrated by transient expression in grape protoplasts. The resistance to the causal agent of downy mildew, Plasmopara viticola, was enhanced in the transgenic plants. These results show that VvWRKY1 can increase resistance of grapevine against the downy mildew through transcriptional reprogramming leading to activation of the jasmonic acid signalling pathway.Keywords
This publication has 46 references indexed in Scilit:
- Arabidopsis WRKY33 Is a Key Transcriptional Regulator of Hormonal and Metabolic Responses toward Botrytis cinerea InfectionPlant Physiology, 2012
- The jasmonate pathway mediates salt tolerance in grapevinesJournal of Experimental Botany, 2012
- Hormone Crosstalk in Plant Disease and Defense: More Than Just JASMONATE-SALICYLATE AntagonismAnnual Review of Phytopathology, 2011
- Phosphorylation of the Nicotiana benthamiana WRKY8 Transcription Factor by MAPK Functions in the Defense ResponsePlant Cell, 2011
- A transcriptomic study of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) interaction with the vascular ascomycete fungus Eutypa lataJournal of Experimental Botany, 2010
- General and species-specific transcriptional responses to downy mildew infection in a susceptible (Vitis vinifera) and a resistant (V. riparia) grapevine speciesBMC Genomics, 2010
- Top hits in contemporary JAZ: An update on jasmonate signalingPhytochemistry, 2009
- A Pair of Allelic WRKY Genes Play Opposite Roles in Rice-Bacteria InteractionsPlant Physiology, 2009
- The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phylaNature, 2007
- The plant immune systemNature, 2006