SPERM DNA FRAGMENTATION
- 1 January 2006
- journal article
- review article
- Published by Informa UK Limited in Archives of Andrology
- Vol. 52 (3), 197-208
- https://doi.org/10.1080/01485010500503561
Abstract
Several techniques have been developed to measure the amount of sperm DNA damage in an effort to identify more objective parameters for evaluation of infertile men. The integrity of sperm DNA influences a couple's fertility and helps predict the chances of pregnancy and its successful outcome. The available tests of sperm DNA damage require additional large-scale clinical trails before their integration into routine clinical practice. The physiological/molecular integrity of sperm DNA is a novel parameter of semen quality and a potential fertility predictor. Although DNA integrity assessment appears to be a logical biomarker of sperm quality, it is not being assessed as a routine part of semen analysis by clinical andrologists. Extensive investigation has been conducted for the comparative evolution of these techniques. However, some of these techniques require expensive instrumentation for optimal and unbiased analysis, are labor intensive, or require the use of enzymes whose activity and accessibility to DNA breaks may be irregular. Thus, these techniques are recommended for basic research rather than for routine andrology laboratories. Sperm chromatin structure evaluation is applied to detect male factors that may affect the chance of success with IVF as well as natural fertility. Further research is needed to define the optimal test of sperm chromatin structure. The clinical application of this test will evolve as well.Keywords
This publication has 75 references indexed in Scilit:
- Sperm DNA damage assessment: a test whose time has comeFertility and Sterility, 2005
- Role of sperm chromatin abnormalities and DNA damage in male infertilityHuman Reproduction Update, 2003
- Role of reactive oxygen species in the pathophysiology of human reproductionFertility and Sterility, 2003
- Telomere Length in Male Germ Cells Is Inversely Correlated with Telomerase Activity1Biology of Reproduction, 2000
- A critical role for telomeres in suppressing and facilitating carcinogenesisCurrent Opinion in Genetics & Development, 2000
- Fertilizing ability of DNA-damaged spermatozoaJournal of Experimental Zoology, 1999
- The extragenomic action of progesterone on human spermatozoa is influenced by redox regulated changes in tyrosine phosphorylation during capacitationMolecular and Cellular Endocrinology, 1996
- Aberrant protamine 1/protamine 2 ratios in sperm of infertile human malesCellular and Molecular Life Sciences, 1988
- Heterogeneity of Sperm Nuclear Chromatin Structure and its Relationship to Bull Fertility1Biology of Reproduction, 1987
- A model for the structure of chromatin in mammalian sperm.The Journal of cell biology, 1982